Previous animal studies have shown differences in CEST properties of radio-resistant and radio-sensitive renal cell carcinoma xenografts. The current study probed the differences in CEST and MT properties of brain metastases from radio-resistant and radio-sensitive primary tumours. We observed significantly lower amount of magnetization transfer, RM0B/RA, and direct water saturation effect, 1/(RAT2A) in brain metastases from radio-resistant tumours compared to those from radio-sensitive tumours. However, the CEST effects of the two cohorts were not different. Such information should be considered when investigating brain metastases and their response to treatment.