Short-range association fibers (SAFs), linking adjacent cortical regions, are dominant in structural connectome and associated with autism and schizophrenia. However, SAFs are not well characterized due to challenges in high-throughput tracing of SAF with diffusion MRI and challenges of identifying and labeling reproducible SAFs. The vast amount of SAFs also make it difficult to delineate them. To meet these challenges, we established a protocol “STTAR” including high-throughput streamline tracing with a regularized FDT probabilistic tractography and semi-automatic identification of reproducible SAFs with novel HDBSCAN clustering. Newly identified reproducible SAFs and those consistently reported in the literature are also demonstrated.