Abstract #0903

Investigating cerebral energetics and neurotransmission using in vivo 1H MRS and [6,6′-2H2]glucose in a preclinical model

Puneet Bagga¹, Laurie J Rich¹, Neil E Wilson¹, Mark Elliott¹, Mitch D Schnall¹, Mohammad Haris^2,3, John A Detre⁴, and Ravinder Reddy¹

¹Department of Radiology, University of Pennsylvania, Philadelphia, PA, United States, ²Sidra Medicine, Doha, Qatar, ³LARC, Qatar University, Doha, Qatar, ⁴Department of Neurology, University of Pennsylvania, Philadelphia, PA, United States

Energy metabolism and neurotransmission are two crucial processes affecting almost all aspects of cerebral function and ¹H MRS allows the non-invasive detection and quantification of neurochemicals. In this study, we performed ¹H MRS in conjuction with the administration of [6,6′-²H₂]glucose to measure turnover kinetics of glutamate, glutamine and GABA in rat brain. As ²H is invisible on ¹H MRS, the turnover of metabolites leads to a corresponding drop in their ¹H MR signal visualized by subtraction of the Post-[6,6′-²H₂]glucose administration from the Pre-administration ¹H MR spectra. The fractional enrichment data can be fitted to evaluate the rates of cerebral energetics.

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