Magnetic Resonance fingerprinting (MRF) provides simultaneous acquisition of T1 and T2 values with high reliability. However, the reproducibility and repeatability of human brain morphometry based on MRF still requires investigation. Here, we examined the feasibility of three-dimensional (3-D) MRF to evaluate the brain cortical thickness and volumetric analysis in healthy volunteers. Scan-rescan tests of both 3-D MRF and conventional 3D T1-weighted imaging were performed. For each sequence, the regional cortical thickness and volume of the subcortical structures were measured using automatic brain segmentation software. High agreement between conventional scans and scan-rescan repeatability in healthy human brains were observed.