We demonstrate the first in vivo imaging experiment with a multiphoton excitation technique at 1T. To produce multiphoton excitation, we built a secondary RF coil that produces an RF field, parallel to the $$$B_{0}$$$ field. Designed for kHz frequencies, this coil consists with two layers of traces in a spiral configuration on a printed circuit board (PCB). Adding this low-frequency coil to an Aspect 1T Wrist Scanner, we acquired gradient echo images with two-photon excitation of a human hand in vivo.
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