Due to the complexity of the heart’s geometry, the characterization of the material properties of the myocardium remains challenging. Using kinematically admissible hyperboloid virtual fields and a finite element mesh of the left ventricle, we successfully solved the virtual field method equations to determine the stiffness of the myocardium from a reverse identification approach. The distribution of the elastic stiffness presented same patterns between the 3 volunteers. However, the standard risk volunteer presented higher stiffness within the mobile wall than the high risk volunteers. This approach would be an efficient tool to characterize early cardiac dysfunction.