Bowel movement is a source of motion artifacts in mouse abdominal MRI. This work aimed to evaluate fasting and hyoscine butilbromide (Buscopan®) for its reduction. Thirteen mice were imaged in a 9.4T MRI scanner with a FLASH sequence for 90 minutes (~19s temporal resolution): 6 were injected with a Buscopan® bolus, 4 with a bolus and constant infusion, and 3 were food deprived. A single bolus of Buscopan® was effective for bowel movement reduction in mice, for up to 1h, whereas other methods were not effective. These data favor the use of Buscopan® for abdominal MRI in the mouse.