The acidification of musculoskeletal tissues is being investigated as a way to localize pain in osteoarthritis. AcidoCEST-UTE MRI can measure pH in tissues such as cartilage and meniscus, though in vivo validation is difficult. Thus, it is imperative that the acidoCEST-UTE sequence be reproducible and verifiable ex vivo, where pH measurements can be confirmed with a pH electrode. This study tested the reproducibility of the acidoCEST-UTE sequence in three ex vivo phantoms, confirming the pH sensitivity of the technique and demonstrating the consistency of the method and results.
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