Abstract #2816

Immersion of ex vivo Achilles tendon in phosphate buffered saline influences their T1 and T2* relaxation times

Martin Krämer¹, Matthias R Kollert^2,3, Nicholas M Brisson², Marta B Maggioni¹, Georg N Duda^2,3, and Jürgen R Reichenbach¹

¹Medical Physics Group, Institute of Diagnostic and Interventional Radiology, Jena University Hospital - Friedrich Schiller University Jena, Jena, Germany, ²Julius Wolff Institute and Center for Musculoskeletal Surgery, Charité – Universitätsmedizin Berlin, Berlin, Germany, ³Berlin-Brandenburg Center and School for Regenerative Therapies, Charité – Universitätsmedizin Berlin, Berlin, Germany

Controlling tissue integrity and hydration levels is a crucial step when preparing and measuring ex vivo samples of tendons. In this work, we immersed ex vivo Achilles tendons in phosphate buffered saline solution and measured T₁ and T_²* relaxation times at baseline, 30 h and 67 h after immersion using 3D ultra-short echo-time imaging with variable flip angles and echo-train shifted multi-echo acquisition, respectively. Results based on regions-of-interest in mid-tendon and enthesis areas showed a significant increase in both T₁ and T₂* after 30 h of immersion in the phosphate buffered saline solution.

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