Abstract #2934

Metabolomic profiling of wildtype and transgenic Giardia lamblia strains by 1H Magic Angle Spinning NMR spectroscopy.

Martina Vermathen¹, Norbert Mueller², David Leitsch³, Damian Hertig⁴, Peter Vermathen⁴, and Joachim Mueller²

¹Department of Chemistry and Biochemistry, University Bern, Bern, Switzerland, ²Institute of Parasitology, Vetsuisse Faculty, University Bern, Bern, Switzerland, ³Institute of Specific Prophylaxis and Tropical Medicine, Medical University of Vienna, Vienna, Austria, ⁴DBMR & DIPR, University Bern, Bern, Switzerland

The intestinal protozoan parasite Giardia lamblia is a major cause of persistent diarrhea in humans and animals worldwide. Treatment of giardiasis with nitro-prodrugs relies on intrinsic enzymes in G.lamblia that are involved in activating or deactivating the prodrugs. To elucidate more about the physiologic function of these pathways and their potential roles in drug resistance, G.lamblia wildtype and transgenic strains were metabolomically characterized based on ¹H HR-MAS NMR data and specific metabolites that may be related to nitroreductase activity were identified and discussed.

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