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Abstract #3131

The cellular heat shock response monitored by chemical exchange saturation transfer MRI

Dennis Kleimaier1, Steffen Goerke2, Cordula Nies3, Moritz Zaiss4, Patrick Kunz5, Eric Gottwald3, and Lothar R. Schad1
1Computer Assisted Clinical Medicine, Heidelberg University, Mannheim, Germany, 2Division of Medical Physics in Radiology, German Cancer Research Center (DKFZ), Heidelberg, Germany, 3Institute of Functional Interfaces, Karlsruhe Institut of Technology, Karlsruhe, Germany, 4Neuroradiology, University of Erlangen-Nürnberg, Erlangen, Germany, 5Division of Functional Genome Analysis, German Cancer Research Center (DKFZ), Heidelberg, Germany

Chemical exchange saturation transfer of the relayed nuclear Overhauser effect(rNOE) enables detection of mobile protein protons via their exchange with the water signal. Recent studies have shown a close relationship between the rNOE-signal and the protein conformation. This study used the -3.5ppm rNOE-signal to monitor the heat shock response of HepG2 cells in a microcavity array-based bioreactor system. A significant drop of the rNOE-signal to 92.4±1.3% and a slow, steady increase of the rNOE-signal for 136.1±13.6min after heat shock were observed. Therefore, the rNOE-signal in CEST spectroscopic imaging is a sensitive readout for the cellular heat shock response.

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