Abstract #3131

The cellular heat shock response monitored by chemical exchange saturation transfer MRI

Dennis Kleimaier¹, Steffen Goerke², Cordula Nies³, Moritz Zaiss⁴, Patrick Kunz⁵, Eric Gottwald³, and Lothar R. Schad¹

¹Computer Assisted Clinical Medicine, Heidelberg University, Mannheim, Germany, ²Division of Medical Physics in Radiology, German Cancer Research Center (DKFZ), Heidelberg, Germany, ³Institute of Functional Interfaces, Karlsruhe Institut of Technology, Karlsruhe, Germany, ⁴Neuroradiology, University of Erlangen-Nürnberg, Erlangen, Germany, ⁵Division of Functional Genome Analysis, German Cancer Research Center (DKFZ), Heidelberg, Germany

Chemical exchange saturation transfer of the relayed nuclear Overhauser effect(rNOE) enables detection of mobile protein protons via their exchange with the water signal. Recent studies have shown a close relationship between the rNOE-signal and the protein conformation. This study used the -3.5ppm rNOE-signal to monitor the heat shock response of HepG2 cells in a microcavity array-based bioreactor system. A significant drop of the rNOE-signal to 92.4±1.3% and a slow, steady increase of the rNOE-signal for 136.1±13.6min after heat shock were observed. Therefore, the rNOE-signal in CEST spectroscopic imaging is a sensitive readout for the cellular heat shock response.

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