Conductivity tensor can realize volume conductor model of brain for neuroimaging and electrical stimulation. We report validation of electrodeless conductivity tensor imaging (CTI) method . From CTI imaging using giant vesicle suspension at 9.4T MRI, relative error in conductivity tensor image was found to be less than 1.7% compared with the measured values using an impedance analyzer. High- and low-frequency conductivity can quantify total and extracellular water contents, respectively, at every pixel. Their difference can quantify intracellular water content at every pixel. Current CTI method can separately quantify the contributions of ion concentrations and mobility to the conductivity tensor.