In vivo MR elastography (MRE) allows non-invasive estimation of aortic stiffness. Recently, the feasibility of multi-slice free-breathing (FB) spin-echo echo-planar imaging (SE-EPI) aortic MRE was investigated. In this study, a single breath-hold (SBH) SE-EPI aortic MRE protocol was proposed and compared to FB SE-EPI aortic MRE. Moreover, in vivo FB and SBH SE-EPI aortic MRE were studied using both1.5T and 3T scanners. The main aim was to assess inter-scanner reproducibilities of FB and SBH SE-EPI aortic MRE.