Delineating the region of interest manually to obtain the magnetic susceptibility values from quantitative susceptibility mapping (QSM) for diagnosis of neurological diseases is a common method. However, this approach is time-consuming and may be inaccurate due to human error. Here we introduced a standardized brain region susceptibility quantification procedure based on QSM and Brainnetome Atlas. Our results show that this method has high accuracy in measuring brain iron content. Based on this method, we found that the content of brain iron tends to increase with age. Additionally, magnetic susceptibility values of partial brain regions have sex differences should be noted.