Current methods for perfusion characterization are difficult to quantify absolutely and provide only relative and qualitative information. Perfusion-phantoms that enable quantitative analysis of transport phenomena are needed to test theoretical models against experimental data. The initial design and deployment of a 3D-printed phantom with pump-driven perfusion of multi-level microvascular structure encapsulated in hydrogel is presented. It simulates vascularized tissue and enables experimental validation. A new quantitative analytical method , using voxelized constitutive convection-diffusion equations, is applied to DCE data and compared to traditional Kety’s methods. The largely qualitative and unmeasurable Kety global AIF assumption is replaced with measurable and reproducible data.