We present initial results of line-scanning fMRI in humans. The potential of this technique lies in the combination of both high spatial and temporal resolution while sacrificing spatial coverage outside the region of interest. We reached a 250 μm resolution along the line direction with a temporal resolution of 200 ms. Coil sensitivity profiles and the average tSNR per channel were used to optimize the line reconstructions. We obtained similar BOLD sensitivity compared to standard 2D GE-EPI BOLD and high spatial specificity for a visual task. Hence, we demonstrate the feasibility of ultra-high spatiotemporal resolution in humans using line-scanning.