Osteosarcoma (OS) is the most common primary malignant bone tumor in children and adolescents. Monitoring treatment response during chemotherapy might help in better and personalized therapeutic options improving overall therapeutic outcome. RECIST1.1 is the standard imaging based non-invasive treatment response evaluation criteria in solid tumors. Quantitative Intravoxel incoherent motion (IVIM) parameters and their histogram analysis were performed in Osteosarcoma in characterizing chemotherapy response with respect to RECIST1.1 criteria. IVIM parameters and its histogram analysis revealed clinically useful information in characterizing chemotherapy response in Osteosarcoma.