Hemodynamic parameters from 4D flow datasets derived in the last years have shown promising diagnostic value in different cardiovascular pathologies. However, we know little about the behavior of these parameters when the 4D flow data is corrupted by respiratory motion. The purpose of this work is to perform a quantitative comparison between hemodynamic parameters computed from 4D flow Cardiac MRI with and without respiratory self-gating. We found significant variability of the hemodynamic parameters in the ascending aorta of healthy volunteers when comparing both methods. Hemodynamic parameters measured with self-gating acquisition showed statistically significant differences compared to those measured without self-gating.