Abstract #0653

Correlating advanced MRI and histopathological measurements of axons and myelin in human traumatic spinal cord injury

Sarah Rosemary Morris^1,2,3, Andrew Yung^1,2,4, Valentin Prevost^1,2,4, Shana George¹, Andrew Bauman^1,2,4, Piotr Kozlowski^1,2,3,4, Farah Samadi^1,5, Caron Fournier^1,5, Lisa Parker⁶, Kevin Dong¹, Femke Streijger¹, Veronica Hirsch-Reinshagen^1,5,6, G.R. Wayne Moore^1,5,6, Brian K Kwon^1,7, and Cornelia Laule^1,2,3,5

¹International Collaboration on Repair Discoveries (ICORD), Vancouver, BC, Canada, ²Radiology, University of British Columbia, Vancouver, BC, Canada, ³Physics & Astronomy, University of British Columbia, Vancouver, BC, Canada, ⁴UBC MRI Research Centre, Vancouver, BC, Canada, ⁵Pathology & Laboratory Medicine, University of British Columbia, Vancouver, BC, Canada, ⁶Vancouver General Hospital, Vancouver, BC, Canada, ⁷Vancouver Spine Surgery Institute, Vancouver, BC, Canada

Using human spinal cord tissue donated to the International Spinal Cord Injury Biobank, we quantitatively correlated binarized histological stains for myelin and axons with myelin- and axon-sensitive advanced MRI metrics (myelin water fraction (MWF), inhomogeneous magnetization transfer (ihMT), diffusion tensor imaging (fractional anisotropy (FA), axial diffusivity (AD), radial diffusivity, RD), diffusion basis spectrum imaging (fibre fraction, FF)). MWF, ihMT and RD had significant, moderately strong correlations with Luxol fast blue staining for myelin phospholipids. FA, AD and FF did not have any significant correlation with phosphorylated neurofilament immunohistochemistry.

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