Recently, diffusion MRI and tractography in musculoskeletal system has used to investigate the tissue microstructure, local collagen fiber alignment, and the 3D collagen network. To the best of our knowledge, nondestructively probing the local collagen fiber direction and 3D collagen fiber architecture is still limited. The water diffusion properties derived from DTI were quantified at different areas of meniscus using the proposed automatic segmentation method. Combining tractography and automatic segmentation, we were able to observe the structural connections among different areas of the meniscus.
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