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Abstract #1463

Mapping of intracellular pH in vivo using amide and guanidyl CEST-MRI at 9.4 T

Philip S Boyd1, Johannes Breitling1, Stephanie Laier2, Karin Mueller-Decker2, Andrey Glinka3, Mark E Ladd1, Peter Bachert1, and Steffen Goerke1
1Division of Medical Physics in Radiology, German Cancer Research Center (DKFZ), Heidelberg, Germany, 2Center for Preclinial Research, Core Facility Tumor Models, German Cancer Research Center (DKFZ), Heidelberg, Germany, 3Division of Molecular Embryology, German Cancer Research Center (DKFZ), Heidelberg, Germany

In this study, we present a method for mapping intracellular pH values based on amide and guanidyl CEST-MRI which simultaneously compensates for concentration changes, the semi-solid magnetization transfer, and spillover dilution. Compensation of the concomitant effects and calculation of reliable pH maps in the range of pH 6.5-7.5 was verified experimentally in porcine brain lysates. Applicability in vivo was demonstrated in the lesions of three tumor-bearing mice showing an average intracellular pH of approximately 7. Consequently, endogenous amide and guanidyl signal-based mapping of the intracellular pH is now applicable for tumor imaging which was previously prevented by the concomitant effects.

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