Amide proton transfer weighted (APTw) imaging is a novel molecular imaging technique to acquire the proton exchanged signals from the amide in proteins or peptides to water. Previous studies discover lateralization in the brain structure and function, including limbic system. However, it is not clear whether APTw also has lateral advantage. In this prospective study, we applied the automatic brain segmentation method to quantify the APTw signal values of limbic system in young healthy subjects. Significant differences of APTw signals between left and right brain hemisphere were found, which may suppose related with right-handedness.