Blood-brain barrier (BBB) is crucial to prevent brain tissue from circulating toxins while allowing the delivery of nutrients from intravascular space to the central nervous system (CNS). Compromised BBB can result in brain dysfunction and degeneration. Development of reproducible non-invasive techniques to assess BBB permeability is of particular interest. Previously we had demonstrated a non-invasive technique to estimate BBB permeability using magnetization transfer (MT) effect on labeled arterial spins at 7T. In this work, we demonstrate the reproducibility of the technique. The feasibility was evaluated in healthy subjects at baseline and after caffeine challenge.