The presence of microvascular invastion (MVI) at histopathology is an important predictive factor in managing hepatocellular carcinoma (HCC). We evaluated the potential of MRE-based shear strain mapping to assess adhesion characteristics at tumor boundaries and to non-invasively predict the presence of MVI. We retrospectively calculated maps displaying octahedral shear strain in exams of 61 HCC patients who underwent 3D multi-frequency MRE examinations. Results demonstrated that the prevalence of adherent tumor-liver parenchyma interface in positive MVI groups was significantly higher than negative groups. The results provide evidence that MRE-based shear strain mapping is helpful for non-invasive prediction of MVI in HCC.