Non-invasive focused ultrasound (FUS) offered attractive advantages to modulate neuronal activity. The functional connectivity (FC) between different brain regions is a dynamic process during a period of examinations. We aim to explore FUS-induced neuromodulation by dynamic FC. We observed the evolution of dynamic FC at Pre-FUS, FUS-35min, and FUS-3hr for normal rats with FUS sonication at ventral posteromedial and ventral posterolateral thalamic nuclei (VPM/VPL) region in left thalamus. With K-means clustering, we quantitatively evaluated the evolution of altered probability% of dynamic FC in seven states at Pre-FUS, FUS-35min, and FUS-3hr, suggesting the potential of FUS-neuromodulation.