Hemifacial spasm (HFS) is a motor disorder. The purpose of this study was to explore the alterations of the cortical-striatal network in HFS using resting-state fMRI. We found that the functional connectivity (FC) between the putamen and ventral striatum and both motor and emotion-related cortex was increased in patients with HFS compared to the healthy controls. The FC between the ventral striatum and the motor cortex was positively correlated with the spasm intensity. To sum up, HFS may lead to altered neural activity in the cortical-striatal loop.