Surgical planning of rotator cuff tears does not benefit from quantitative measurement of muscles stiffness and microstructure leading to retears in the following years. Since stiffness is dependent on anisotropy, Diffusion Tensor Imaging can simplify the inversion algorithm of Magnetic resonance elastography. Repeatability of DTI metrics from three scans in six shoulders of six asymptomatic volunteers was achieved (coefficient of variance <10%) for the fractional anisotropy, mean diffusivity, mode and eigenvalues. Clear convergence of myocytes to the tendons was observed with tractography in the supraspinatus as known in dissection. This MRI protocol is promising for developing biomarkers for surgical planning.