Layer specific MR properties have previously been observed in the superior colliculus in vivo at 9.4T. The present study investigates layer specific MR properties in R2* and R1 in more detail in post mortem brain stem samples at 14.1T. Similar to in vivo, one R2* maximum that likely corresponds to the superficial optic layer (layer III) was observed. Ex vivo we could observe high R2* and R1 in an area likely corresponding to the deep white layer (layer VII) of the superior colliculus. In the R1maps, this area showed a strong contrast towards the periaqueductal gray.