In this study, we report on the reproducibility of DTI-derived metrics w.r.t. the phase-encoding directions (PED) used for the acquisition of diffusion weighted images. Over the entire brain, the reproducibility of DTI metrics was higher for data acquired using LR/RL phase-encoding directions. However, AP/PA data showed better reproducibility in some regions. The main source of these reproducibility variations was identified as ghosts overlapping with different brain regions depending on PED. We conclude that acquiring data with all four phase-encoding directions would be the most beneficial to achieve maximum reproducibility in all brain regions after proper editing of regionally corrupted data.
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