Meeting Banner
Abstract #3594

Comparing aspartate and bicarbonate produced from hyperpolarized 1-13C pyruvate as markers of renal gluconeogenic flux

Hikari A. I. Yoshihara1, Arnaud Comment2,3, and Juerg Schwitter4,5
1Laboratory for Functional and Metabolic Imaging, Institute of Physics, Swiss Federal Institute of Technology, Lausanne (EPFL), Lausanne, Switzerland, 2Cancer Research UK Cambridge Institute, University of Cambridge, Cambridge, United Kingdom, 3General Electric Healthcare, Chalfont St Giles, United Kingdom, 4Division of Cardiology, Lausanne University Hospital (CHUV), Lausanne, Switzerland, 5Cardiac MR Center, Lausanne University Hospital (CHUV) and University of Lausanne (UNIL), Lausanne, Switzerland

Labeled metabolites of hyperpolarized 1-13C pyruvate, including aspartate, malate and fumarate are detected in the rat kidney in vivo, indicating the possibility of detecting gluconeogenic flux. Pyruvate-to-bicarbonate conversion in the fasted rat liver is a marker of PEP-CK flux. Fasting resulted in lower bicarbonate and higher aspartate in the kidney. Conversely, treatment with the PEP-CK inhibitor 3-MPA did not affect bicarbonate production but did yield a lower normalized aspartate signal, with a 12-fold reduction in fasted rats. Renal pyruvate-to-bicarbonate conversion is therefore largely attributable to pyruvate hydrogenase flux, while pyruvate-to-aspartate conversion is a potential marker of renal gluconeogenesis.

This abstract and the presentation materials are available to members only; a login is required.

Join Here