Abstract #3594

Comparing aspartate and bicarbonate produced from hyperpolarized 1-13C pyruvate as markers of renal gluconeogenic flux

Hikari A. I. Yoshihara¹, Arnaud Comment^2,3, and Juerg Schwitter^4,5

¹Laboratory for Functional and Metabolic Imaging, Institute of Physics, Swiss Federal Institute of Technology, Lausanne (EPFL), Lausanne, Switzerland, ²Cancer Research UK Cambridge Institute, University of Cambridge, Cambridge, United Kingdom, ³General Electric Healthcare, Chalfont St Giles, United Kingdom, ⁴Division of Cardiology, Lausanne University Hospital (CHUV), Lausanne, Switzerland, ⁵Cardiac MR Center, Lausanne University Hospital (CHUV) and University of Lausanne (UNIL), Lausanne, Switzerland

Labeled metabolites of hyperpolarized 1-¹³C pyruvate, including aspartate, malate and fumarate are detected in the rat kidney in vivo, indicating the possibility of detecting gluconeogenic flux. Pyruvate-to-bicarbonate conversion in the fasted rat liver is a marker of PEP-CK flux. Fasting resulted in lower bicarbonate and higher aspartate in the kidney. Conversely, treatment with the PEP-CK inhibitor 3-MPA did not affect bicarbonate production but did yield a lower normalized aspartate signal, with a 12-fold reduction in fasted rats. Renal pyruvate-to-bicarbonate conversion is therefore largely attributable to pyruvate hydrogenase flux, while pyruvate-to-aspartate conversion is a potential marker of renal gluconeogenesis.

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