Diffusion tensor imaging (DTI) is a functional imaging technology improved and developed on the basis of DWI technology. It reflects the changes of free diffusion rate and direction of water molecules caused by different structures in living tissues. It can obtain multiple quantitative parameters through post-processing. There is no report on the evaluation of endometrial carcinoma (EC) microsatellite instability (MSI) status by DTI quantitative parameters. Results shown that multiple quantitative parameters of DTI were different between MSI and microsatellite stability (MSS) of EC, therefore, it is feasible to use DTI for predicting MSI of EC.