It is difficult to differentiate prostate tumors and prostate hyperplasia using conventional MR imaging methods. Amide proton transfer-weighted imaging is a novel MRI imaging tool for detection of amide protons in mobile cellular proteins and peptides. We aimed to evaluate the differential diagnosis of prostate cancers and benign prostatic hyperplasia using APT and IVIM. Results showed that prostate cancers were associated with higher APT values than benign prostate hyperplasia, while diffusion and perfusion parameters of IVIM were lower in prostate cancers. The diagnostic efficiency between prostate cancer and prostatic hyperplasia was higher with combinational use of APT and IVIM parameters.