Meeting Banner
Abstract #0511

DEXSY can measure water exchange linked to cellular homeostasis and active states in central nervous system tissue

Nathan H. Williamson1,2, Rea Ravin1, Teddy X. Cai1,3, Melanie Falgairolle4, Michael J. O'Donovan4, and Peter J. Basser1
1National Institute of Child Health and Human Development, Potomac, MD, United States, 2National Institute of General Medical Sciences, Potomac, MD, United States, 3Department of Clinical Neurosciences, Wellcome Centre for Integrative Neuroimaging, FMRIB, University of Oxford, Oxford, United Kingdom, 4National Institute of Neurological Disorders and Stroke, Potomac, MD, United States


We propose a new functional MR method based on steady-state transmembrane water exchange rate measurements with diffusion exchange spectroscopy (DEXSY). Rapid DEXSY methods are implemented on a low-field MR system with a strong static gradient to test for an active component of water exchange in ex vivo neonatal mouse spinal cords. Temperature-dependent Arrhenius activation energies for water exchange are significantly greater in viable “live” samples than in the same samples after fixation, suggesting a connection to ATP-driven enzymatic processes. Moreover, exchange rates in live samples significantly decrease after blocking Na+/K+-ATPase pump activity, revealing an active component of water exchange.

This abstract and the presentation materials are available to members only; a login is required.

Join Here