Abstract #0702

Detection of Laser-Associated Heating in the Brain During Simultaneous BOLD-fMRI and Optogenetic Stimulation

Huiwen Luo^1,2, Zhangyan Yang^1,2, Pai-feng Yang^2,3, Feng Wang^2,3, Jamie L Reed^2,3, Li Min Chen^2,3, John C Gore^1,2,3, and William A Grissom ^1,2,3

¹Biomedical Engineering, Vanderbilt University, Nashville, TN, United States, ²Vanderbilt University Institute of Imaging Science, Vanderbilt University, Nashville, TN, United States, ³Radiology and Radiological Sciences, Vanderbilt University, Nashville, TN, United States

Synopsis

Optogenetic stimulation with BOLD-fMRI has been applied in studies of brain circuits, by selectively exciting optical fluorophores with wavelength-specific light. However, the light used for stimulation may induce non-negligible heating effects. Because BOLD fMRI is mainly based on relatively long TE gradient-recalled echo acquisitions that are also suitable for measuring temperature changes with the proton resonance frequency (PRF) shift, we can monitor simultaneously the neural effects and laser-associated heating. We proposed a processing pipeline to calculate temperatures from BOLD images based on PRF shift thermometry and detect small temperature rises induced by laser-associated heating with general linear modeling.

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