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Abstract #0702

Detection of Laser-Associated Heating in the Brain During Simultaneous BOLD-fMRI and Optogenetic Stimulation

Huiwen Luo1,2, Zhangyan Yang1,2, Pai-feng Yang2,3, Feng Wang2,3, Jamie L Reed2,3, Li Min Chen2,3, John C Gore1,2,3, and William A Grissom 1,2,3
1Biomedical Engineering, Vanderbilt University, Nashville, TN, United States, 2Vanderbilt University Institute of Imaging Science, Vanderbilt University, Nashville, TN, United States, 3Radiology and Radiological Sciences, Vanderbilt University, Nashville, TN, United States


Optogenetic stimulation with BOLD-fMRI has been applied in studies of brain circuits, by selectively exciting optical fluorophores with wavelength-specific light. However, the light used for stimulation may induce non-negligible heating effects. Because BOLD fMRI is mainly based on relatively long TE gradient-recalled echo acquisitions that are also suitable for measuring temperature changes with the proton resonance frequency (PRF) shift, we can monitor simultaneously the neural effects and laser-associated heating. We proposed a processing pipeline to calculate temperatures from BOLD images based on PRF shift thermometry and detect small temperature rises induced by laser-associated heating with general linear modeling.

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