Pulse wave velocity (PWV) is a non-invasive biomarker related to vascular stiffness and cardiovascular risk. Cine 2D phase contrast (2DPC) MRI can be used to assess aortic PWV. However, the effects of respiration on PWV measures remain unexplored. Here, we assessed the effects of respiration by retrospectively gating free breathing radially sampled 2DPC MRI data in both inspiration and expiration. Two axial slices were obtained in the aortic arch and abdominal aorta and time shift methods were used to calculate PWV between the ascending and abdominal aorta. PWV measurements from inspiration (7.58m/s) were significantly decreased relative to expiration (8.42m/s).