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Abstract #1015

Serial in vivo 19F MRI tracks the immune cell response to bioscaffolds implanted in a rat model of stroke

Mike Modo1, Harmanvir Ghuman2, Reem Azar2, Ryan Krafty3, Stephen Badylak4, and T. Kevin Hitchens5
1Radiology, University of Pittsburgh, Pittsburgh, PA, United States, 2Bioengineering, University of Pittsburgh, Pittsburgh, PA, United States, 3Biological Science, University of Pittsburgh, Pittsburgh, PA, United States, 4Surgery, University of Pittsburgh, Pittsburgh, PA, United States, 5Neurobiology, University of Pittsburgh, Pittsburgh, PA, United States

Synopsis

Regenerative medicine is increasingly investigating how an initial pro-inflammatory response convert to a pro-repair response. Implantation of bioscaffolds exploits this mechanism and allows tissue to regrow in large defects. Even the brain, which is known to not regrow tissue spontaneously, can regenerate lost tissue after implantation of a inductive bioscaffold. Little is known about how immune cells respond to the implantation of a bioscaffold. We here characterized the acute spatiotemporal dynamics of immune cell infiltration into the brain and a bioscaffold by tagging immune cells with perfluorocarbon nanoemulsions for detection by 19F MRI and anatomical context using 1H MRI.

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