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Abstract #1657

Sample Extraction of ex vivo Myocardial Tissue: Combination of High Resolution MRI with 3D-printed Guides

Simon Reiss1, Johannes Fischer1, Julien Thielmann2, Thomas Lottner1, Timo Heidt2, Constantin von zur Mühlen2, and Michael Bock1
1Dept. of Radiology, Medical Physics, Medical Center University of Freiburg, Faculty of Medicine, University of Freiburg, Freiburg, Germany, 2Dept. of Cardiology and Angiology I, University Hospital Freiburg and Faculty of Medicine, University of Freiburg, Freiburg, Germany

Synopsis

Magnetic resonance provides a multitude of imaging techniques to detect and characterize myocardial infarction (MI). Terminal animal studies are often performed in which CMR findings after MI are correlated to histology. However, precise manual extraction of tissue samples from a specific myocardial region defined by CMR is difficult. Here, we propose the use of 3D-printed guides for extraction of tissue samples from myocardial regions pre-defined on high resolution ex vivo CMR. Using the presented technique, histology findings can be correlated to the exact position within the MRI data set with a precision of < 1 mm.

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Keywords