Some applications require T2-weighted mouse brain MRI with isotropic high resolution (100µm3), but at 9.4T the short T2 of mouse brain limits the echo train length of RARE sequences and exacerbates T2-decay artifacts. Adapting a RARE sequence by using variable flip-angles allows a reduction in scan time from 19min to 7-9min with similar image quality. However, long RARE echo trains and strong imaging gradients can unintentionally cause substantial diffusion weighting. To minimize the diffusion effects crusher gradients were optimized and the variable flip angles were kept above 40°.
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