The robustness of probabilistic versus deterministic DTI tractography in characterization of skeletal muscle structure is not yet established. We investigated the sensitivity to turning angle when probabilistic and deterministic methods were used to estimate fascicle lengths and fractional anisotropy in the arm’s biceps muscle (n=8). Results produced with deterministic methods were highly sensitive to the turning angle, but those produced with probabilistic methods were not. Furthermore, the recommended angle in probabilistic methods yielded findings consistent with ground truth measurements, while the turning angle in deterministic methods needed to be altered from the recommended value to achieve similar results.
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