There has been a long-standing demand for high temporospatial resolution in non-invasive neuroimaging. Using our previously proposed approach for direct imaging of neuronal activity (DIANA-fMRI) with a high temporal resolution of milliseconds, we continued to demonstrate DIANA-fMRI performance in mice in vivo at 9.4T using visual stimulation. The DIANA signal change was significantly increased (~0.2-0.4%) in response to flashing light stimulus, and it was found that DIANA responses of sSC, V1, and V2 were sequentially activated in that order. The DIANA response times of sSC, V1, and V2 were consistent with previous electrophysiological studies.
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