Aquaporin-4 (AQP4) is a transmembrane water channel highly expressed in central nervous system, regulating fluid exchange by water transport between two sides of plasmatic membrane, and depending on concentration gradients of solutes, like glucose. On these grounds, we studied AQP4’s role in glucose uptake. AQP4 inhibitor TGN-020 was administrated in adult mice before vehicle and glucose stimulus. Diffusion and T2* images were acquired and apparent diffusion coefficient and T2* were measured. Ex vivo 1H high-resolution magic angle spinning spectra were acquired. Results showed that TGN avoid physiological cerebral response linked to glucose uptake, noting the AQP4 involvement in the process.