Diffusion and IVIM virtual MR elastography (dMRE) provides quantitative estimates of tissue stiffness without using mechanical vibrations. It rarely applied to identify focal liver lesions (FLL). This retrospective study included 21 subjects (11 malignant lesions and 10 benign lesions). DWI were obtained to calculating dMRE. The kPa values of malignant FLLs (7.93 ± 2.37) was significantly higher than that of benign FLLs (-0.28±5.37) (P < 0.01). ROC showed an AUC of 0.93 for dMRE in differentiating maglinant FLLs and benign FLLs. The sensitivity and specificity were 91% and 90%, respectively. Malignant lesions could be distinguished from benign FLLs by dMRE.