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Abstract #2754

Characterising hypoxia in rhabdomyosarcoma xenografts with oxygen-enhanced MRI

Jessica K.R. Boult1, Upasana Roy1, Carolina Bernauer2, Carol Box1, Louise Howell3, Elise Y. Lepicard1, Yann Jamin1, James P.B. O'Connor1, Janet M. Shipley2, and Simon P. Robinson1
1Division of Radiotherapy and Imaging, The Institute of Cancer Research, London, United Kingdom, 2Division of Molecular Pathology, The Institute of Cancer Research, London, United Kingdom, 3Core Research Facilities, The Institute of Cancer Research, London, United Kingdom


Hypoxic gene signatures are prevalent in paediatric rhabdomyosarcomas and are important in conferring resistance to standard treatments. Oxygen-enhanced MRI (OE-MRI) and histological assessment of pimonidazole adduct formation show that xenografts derived from three human rhabdomyosarcoma cell lines exhibit high levels of hypoxia with differences in vascular perfusion evident between the models.

There is much interest in hypoxia-alleviating strategies to reduce tumour hypoxia for therapeutic gain, and these will be assessed in rhabdomyosarcoma models in vivo using OE-MRI. A reduction in hypoxia in response to atovaquone in spheroids derived from the same rhabdomyosarcoma cell lines in vitro has been confirmed.

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