Punctate white matter lesions (PWMLs) were currently common white matter injuries, with incidence of 20-30% and associated with adverse neurological development. Longitudinal alteration of PWMLs was less researched which might be related to pathology. Therefore, this study aimed to assess alterations of PWMLs virtual shear stiffness in neonates via DKI-based virtual elastography. We found that virtual shear stiffness of PWMLs was higher than contemporaneous white matter at neonatal periods and did not change obviously with age, while virtual shear stiffness of normal white matter was increased with age. Virtual elastography might be a potential method to identify differently nosological PWMLs.