Compared to clinical field strengths, MRI at ultra-high magnetic fields allows higher SNR. However, imaging at 7T remains challenging, especially in renal MRI. This study investigated the feasibility of renal MRI at 5T, with a brand new 5T MR scanner. Compared to 3T examination, 5T renal MRI demonstrated higher SNR and improved corticomedullar discrimination with diagnostic image quality. Functional imaging, including DWI and T2* mapping, was also feasible at 5T. Therefore, in vivo 5T renal MRI may better elucidate the renal diseases with both anatomical and functional imaging, compared to conventional clinical MRI scanners.