Mapping cortical myelination has been a long-standing goal for accurate parcellation and classification of cytoarchitectonic boundaries. We investigated a multivariate myelin mapping approach for the accurate parcellation of small cortical subregions of the insula by exploiting the co-localization of iron with dense bands of myelin in cortical gray matter. We assessed the performance of multivariate myelin mapping in the rhesus macaque, both in vivo and ex vivo, and compared its performance with T1w/T2w ratio imaging. We demonstrated that multivariate myelin mapping is a translatable myelin sensitive technique for the differentiation of insula subregions.
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