Keywords: Spectroscopy, Deuterium, , 2H-MRS, Brain, TCA, MetabolismImpaired neurometabolism is often attributed to altered TCA flux (VTCA). Since, β-hydroxybutyrate metabolism bypasses glycolytic flux, it can be better used to monitor VTCA. In the current study, [3,4,4,4]-2H4-β-hydroxybutyrate was infused in 6-month-old mice, and the level of [4,4]-2H2-Glx was monitored using 2H-MRS. A kinetic model was fitted to determine the rate of BHB metabolism (CMRBHB) and VTCA. Glx labeling followed a sigmoidal curve to reach a quasi-steady state concentration (~1.6±0.1 mM) in 30 minutes. CMRBHB and VTCA were determined to be 0.054±0.004 and 0.12±0.01 µM/g/min. In conclusion, this method can be used to monitor neurometabolism in health and disease.
How to access this content:
For one year after publication, abstracts and videos are only open to registrants of this annual meeting. Registrants should use their existing login information. Non-registrant access can be purchased via the ISMRM E-Library.
After one year, current ISMRM & ISMRT members get free access to both the abstracts and videos. Non-members and non-registrants must purchase access via the ISMRM E-Library.
After two years, the meeting proceedings (abstracts) are opened to the public and require no login information. Videos remain behind password for access by members, registrants and E-Library customers.
Keywords