Abstract #0368

Non-contrast assessment of blood-brain barrier permeability to water in mice: An arterial spin labeling study

Zhiliang Wei^1,2, Hongshuai Liu³, Zixuan Lin¹, Minmin Yao³, Ruoxuan Li³, Chang Liu³, Yuguo Li^1,2, Jiadi Xu^1,2, Wenzhen Duan^3,4, and Hanzhang Lu^1,2,5

¹Russell H. Morgan Department of Radiology and Radiological Science, Johns Hopkins University School of Medicine, Baltimore, MD, United States, ²F. M. Kirby Research Center for Functional Brain Imaging, Kennedy Krieger Research Institute, Baltimore, MD, United States, ³Department of Psychiatry and Behavioral Sciences, Johns Hopkins University School of Medicine, Baltimore, MD, United States, ⁴The Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD, United States, ⁵Department of Biomedical Engineering, Johns Hopkins University School of Medicine, Baltimore, MD, United States

Synopsis

Keywords: Arterial spin labelling, AnimalsBlood-brain barrier (BBB) plays a critical role in brain health and diseases. However, there is a scarcity of tools to assess BBB integrity, particularly in mouse models. Here, we aimed to develop a non-contrast arterial-spin-labeling-based MRI technique to estimate BBB permeability in mice by measuring relative fractions of labeled water in cerebral veins. Systematic optimizations were performed to enhance signal sensitivity with a further study investigating reproducibility. The proposed method revealed significant BBB dysfunctions in a mouse model of Huntington’s disease, which were further validated with histology. Our method may open new avenues for preclinical mechanistic research or therapeutic trails.

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