Keywords: CEST & MT, CEST & MTAcquisition of high-resolution Z-spectra requires excessive scan times. Ultrafast Z-spectroscopy (UFZ) can obtain whole Z-spectra within one acquisition by encoding the Z-spectral dimension spatially via a gradient applied concurrently with the saturation RF pulse, significantly reducing the scanning time. Still, UFZ has had limited success in vivo due to tissue heterogeneity. Here, we developed a TS-UFZ imaging approach where both saturation gradient and its readout were applied in the slice direction, where there is minimal heterogeneity. Results show that TS-UFZ allows high spectral- and spatial-resolution imaging of CEST signals in phantoms and human brain at 3T.
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