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Abstract #1036

Optogenetic modulation of the mouse default mode network with a single tapered fiber

Elizabeth de Guzman1, Barbara Spagnolo2, Filippo Pisano2, Marco Pisanello2, Alberto Galbusera1, Luigi Balasco3, Yuri Bozzi3, Massimo De Vittorio2,4, Tommaso Fellin5, Ferruccio Pisanello2, and Alessandro Gozzi1
1Functional Neuroimaging Lab, Istituto Italiano di Tecnologia, Rovereto, Italy, 2Center for Biomolecular Nanotechnologies, Istituto Italiano di Tecnologia, Arnesano (Lecce), Italy, 3Center for Mind/Brain Sciences (CiMEC), University of Trento, Rovereto, Italy, 4Dipartimento di Ingegneria dell’Innovazione, Università del Salento, Lecce, Italy, 5Optical Approaches to Brain Function Laboratory, Istituto Italiano di Tecnologia, Genova, Italy

Synopsis

Keywords: Brain Connectivity, fMRI, preclinical, functional connectivity, neural oscillations, DMNThe default mode network (DMN) is a distributed functional system of the human brain widely studied with fMRI due to its involvement in advanced cognitive processes and its dysregulation in a variety of brain disorders. Causal perturbations of this network in physiologically accessible species are critically required to probe its circuit organization and the underpinnings of its (dys)function. Here we show that tapered fiber optogenetic technology enables the reliable stimulation of key DMN nodes in a frequency dependent fashion, overcoming the limitations of traditional optogenetic approaches.

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